General Description
The Genome Analyzer IIx offers a powerful combination of 2 x 150 bp read lengths and up to 640 million paired-end reads per flow cell, enabling a broad range of high-throughput sequencing applications. Evidenced by a vast number of peer-reviewed publications in an ever-broadening range of applications, Illumina sequencing technology with the Genome Analyzer IIx is a proven platform for genomic discovery and validation.
Technical Description
Illumina sequencing technology leverages clonal array formation and proprietary reversible terminator technology for rapid and accurate large-scale sequencing. The innovative and flexible sequencing system enables a broad array of applications in genomics, transcriptomics, and epigenomics.
Evaluation Criteria
THROUGHPUT:
- Greater than 8 hours for detection
- Multiple samples, multiple tests/sample per run
- Less than 32 samples every 2 hours
- The system or device is currently semi-automated
- Device or system is intended for multiple detection assays
- 5 or more solutions, buffer, eluents, and/or reagents
- 5 or more components
- Greater than 20 minutes is required for set-up
- Greater than 12 steps are required for detection
LOGISTICS:
- A day of training and technical skills are required
- Larger than a home dishwasher
- More than 50 kg
- Wired connections are available
- System or device has 110V electrical requirement
OPERATIONS:
- Can be used from 25°C to 37°C
- Components must be frozen (-20°C)
- Device or system has peak performance at normal relative humidity conditions
- Between 6 months and 1 year shelf life
- 5-10 years expected life
- Results can be viewed in real-time
- The system is not capable of autonomy
- The system software is open and available for modification
- The system hardware is closed and not available for modification
DETECTION:
- This system does not test liquids
- Excellent specificity. System has occasional false alarms under certain conditions (<2%)
- 100-1,000 CFU per mL
- 100-1,000 PFU per mL
- Manual kit not integrated with the system handles spore lysis
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