HiScanSQ Systems | Illumina, Inc. | WMD Detector Selector

General Description
The HiScanSQ system integrates the power and resolution of next-generation sequencing with the high-throughput capacity of genotyping and gene expression arrays, delivering unprecedented flexibility for experimental design. The instrument features two distinct components, the HiScan Reader and the SQ Module. The HiScan Reader functions as a high-speed, precision imaging scanner for Illumina sequencing and microarray-based analyses. The SQ Module is reagent handling fluidics device needed to perform Illumina next-generation sequencing. Other components include the cBot Cluster Generation System, a suite of data collection and analysis software, and dedicated consumables. cBot provides automated clonal amplification of single molecules randomly distributed on a glass surface.

Technical Description
The HiScan Reader is a high-speed, precision imaging scanner for Illumina sequencing and microarray-based analyses. The SQ Module is an add-on reagent handling fluidics device needed to perform Illumina next-generation sequencing on the HiScan reader. BeadArray technology is utilized in Illumina's HiScanSQ System for a broad range of DNA and RNA analysis applications. Illumina’s BeadArray Technology is based on sub-micron silica beads that self-assemble in microwells on planar silica slides. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in one of Illumina’s assays. Illumina sequencing technology leverages clonal array formation and proprietary reversible terminator technology for rapid and accurate large-scale sequencing. The innovative and flexible sequencing system enables a broad array of applications in genomics, transcriptomics, and epigenomics.

Evaluation Criteria

THROUGHPUT:

Greater than 8 hours for detection
Multiple samples, multiple tests/sample per run
Less than 32 samples every 2 hours
The system or device is currently semi-automated
Device or system is intended for multiple detection assays
5 or more solutions, buffer, eluents, and/or reagents
5 or more components
Greater than 12 steps are required for detection

LOGISTICS:

A day of training and technical skills are required
Larger than a home dishwasher
More than 50 kg
Wired connections are available
System or device has 110V electrical requirement

OPERATIONS:

Can be used from 25°C to 37°C
Components must be frozen (-20°C)
Device or system has peak performance at normal relative humidity conditions
Between 1 to 6 months shelf life
5-10 years expected life
Results can be viewed in real-time
The system is not capable of autonomy
The system software is open and available for modification
The system hardware is open and available for modification

DETECTION:

This system does not test liquids
Excellent specificity. System has occasional false alarms under certain conditions (<2%)
100-1,000 CFU per mL
100-1,000 PFU per mL
Manual kit not integrated with the system handles spore lysis

Tier Selection