HiSeq 2000 Sequencing System | Illumina, Inc.

General Description
The Illumina HiSeq 2000 Sequencing System is an integrated platform that uses massively parallel sequencing technology for genetic analysis and functional genomics. It consists of the HiSeq 2000 sequencer, which uses TDI line scanning and dual surface imaging to generate up to 600 gigabases (Gb) per run and up to 60 Gb per day. Other components include the cBot Cluster Generation System, a suite of data collection and analysis software, and dedicated consumables. cBot provides automated clonal amplification of single molecules randomly distributed on a glass surface. Resulting DNA clusters are sequenced on the HiSeq 2000 using sequencing by synthesis method with patented reversible terminator chemistry.

Technical Description
Illumina sequencing technology leverages clonal array formation and proprietary reversible terminator technology for rapid and accurate large-scale sequencing. The innovative and flexible sequencing system enables a broad array of applications in genomics, transcriptomics, and epigenomics.

Evaluation Criteria

THROUGHPUT:

Greater than 8 hours for detection
Multiple samples, multiple tests/sample per run
Less than 32 samples every 2 hours
The system or device is currently fully automated
Device or system is intended for multiple detection assays
5 or more solutions, buffer, eluents, and/or reagents
5 or more components
Less than 5 minutes is required for set-up
Greater than 12 steps are required for detection

LOGISTICS:

An afternoon of training and some technical skills required
Larger than a home dishwasher
More than 50 kg
Wired connections are available
System or device has 220V electrical requirement

OPERATIONS:

Can be used from 25°C to 37°C
Components must be frozen (-20°C)
Device or system has peak performance at normal relative humidity conditions
Between 6 months and 1 year shelf life
5-10 years expected life
Results can be viewed in real-time
The system is not capable of autonomy
The system software is open and available for modification
The system hardware is closed and not available for modification

DETECTION:

This system does not test liquids
Excellent specificity. System has occasional false alarms under certain conditions (<2%)
100-1,000 CFU per mL
100-1,000 PFU per mL
Manual kit not integrated with the system handles spore lysis

Tier Selection