General Description
The LightCycler 480 Real-Time PCR System is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. Building on the benefits of Roche's capillary-based LightCycler® Systems, it goes one step further in offering enhanced throughput, compatibility with automation equipment and maximum flexibility regarding hard- and software. Providing novel ways to combine speed and accuracy without compromises, the LightCycler® 480 Real-Time PCR System meets the needs of a broad range of applications in research fields such as gene expression studies, discovery and analysis of genetic variation or array data validation.
Technical Description
The LightCycler 480 Real-Time PCR System is a fully integrated multiwell-plate based real-time PCR platform. Benefit from novel thermal cycling and data capture technologies to achieve outstanding temperature homogeneity and assay reproducibility. Increase the sensitivity and yield of your real-time PCR applications, with robust LightCycler® 480 reagents and specially engineered clear or white consumables. Analyze true raw data, breaking free from the need to use passive reference dyes or normalization plates to get accurate results. Flexibly switch between 96- or 384-well plate formats. Select from numerous assay formats and detection dyes, expanding the range of real-time PCR applications in your lab.
Evaluation Criteria
THROUGHPUT:
- Between 30 and 60 minutes for detection
- Multiple samples, multiple tests/sample per run
- Greater than 750 samples every 2 hours
- The system or device is currently fully automated
- Device or system is intended for multiple detection assays
- 0-1 solutions, buffer, eluents, and/or reagents
- 2 components
- 10-20 minutes is required for set-up
- 1-2 steps are required for detection
LOGISTICS:
- Very brief (minutes-hours) training and minimal technical skills
- Approximately the size of a home dishwasher
- More than 50 kg
- Satellite, wireless and wired connections are available
- System or device has 220V electrical requirement
OPERATIONS:
- Can be used from 4°C to 37°C
- Components must be frozen (-20°C)
- Between 1 to 3 years shelf life
- 5-10 years expected life
- Results can be viewed in real-time
- The system is not capable of autonomy
- The system software is closed and not available for modification
- The system hardware is closed and not available for modification
DETECTION:
- Possible the system could receive 510K clearance, no current efforts at this time
- Possible the system could receive FDA approval, no current efforts at this time
- Less than 10 µL
- Superior specificity. System has a false alarm rate approaching zero (~0%)
- 1-100 CFU per mL
- 1-100 PFU per mL
- Less than 1 ng per mL
- Spore lysis not necessary for detection by system