General Description
The Microfluidic - Bioagent Autonomous Networked Detector (M-BAND) is a fully automated, wirelessly networked airborne pathogen detection and identification system designed to run for up to 1 month continuously without human intervention. It can specifically identify bacteria, viruses, and toxins at threat levels indoors and outdoors. It has been field tested for over 2 years by several independent agencies domestically and internationally.
Technical Description
The M-BAND is a modular and fully integrated system that includes a high volume air collector, sample purification, Taqman PCR for bacteria and viruses, and chemiluminescence immunoassays for toxins. It includes 16 multiplexed PCR signatures for 6 bio-threat agents, and immunoassays for 3 toxins. It has extra capacity designed into the system for additional bio-threat agents to be included in detection. It is fully networked and remotely operable.
Evaluation Criteria
THROUGHPUT:
- Between 60 minutes and 8 hours for detection
- 1 sample, >10 tests/sample per run
- Less than 32 samples every 2 hours
- The system or device is currently fully automated
- Device or system is intended for multiple detection assays
- 5 or more solutions, buffer, eluents, and/or reagents
- 1 component
- Greater than 20 minutes is required for set-up
- Automatic detection
LOGISTICS:
- A day of training and technical skills are required
- Larger than a home dishwasher
- More than 50 kg
- Wireless and wired connections are available
- System or device requires multiple outlets or a dedicated circuit breaker
OPERATIONS:
- Can be used from < -21°C to > 42°C (All temperatures)
- Components must be stored at 4°C
- Performance is not influenced by relative humidity
- Between 1 to 3 years shelf life
- 5-10 years expected life
- Results cannot be viewed in real-time
- The system or device is currently fully autonomous
- The system software is open and available for modification
- The system hardware is open and available for modification
DETECTION:
- Greater than 250 µL
- Superior specificity. System has a false alarm rate approaching zero (~0%)
- 100-1,000 CFU per mL
- 100-1,000 PFU per mL
- 1-10 ng per mL
- Fully automated spore lysis
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