General Description
MiSeq is a next generation sequencer design for individual research labs. Key characteristics include rapid turnaround times, a simple workflow from sample prep to data analysis, high accuracy and output in excess of 1Gb.
Technical Description
MiSeq leverages standard molecular biology techniques to yield adapter ligated products that are then loaded onto the MiSeq system. There, the fragments are clonally amplified by bridge amplification and then sequenced using a sequencing by synthesis reversible terminator chemistry. Each based is imaged, processed and reported out as a variant call all on the same instrument.
Notes:
In use at DoD regional laboratories under the Global Biosurveillance Technologies Initiative (GBTI).
Evaluation Criteria
THROUGHPUT:
- Between 60 minutes and 8 hours for detection
- Multiple samples, multiple tests/sample per run
- Less than 32 samples every 2 hours
- The system could be adapted to a semi-automated system with some effort
- Device or system is intended for multiple detection assays
- 2 solutions, buffer, eluents, and/or reagents
- 5 or more components
- 9-12 steps are required for detection
LOGISTICS:
- An afternoon of training and some technical skills required
- Approximately the size of a carry-on luggage suitcase
- More than 50 kg
- Wired connections are available
- System or device has 110V electrical requirement
OPERATIONS:
- Can be used from 25°C to 37°C
- Components must be frozen (-20°C)
- Between 6 months and 1 year shelf life
- 3-5 years expected life
- Results can be viewed in real-time
- The system is not capable of autonomy
- The system software is open and available for modification
- The system hardware is closed and not available for modification
DETECTION:
- This system does not test liquids
- Excellent specificity. System has occasional false alarms under certain conditions (<2%)
- 100-1,000 CFU per mL
- 100-1,000 PFU per mL
- Manual kit not integrated with the system handles spore lysis
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