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Average User Rating:

Applied Biosystems - 7900HT Fast Real-Time PCR System
Contact:

Life Technologies

850 Lincoln Centre Dr MS 407

Foster City, CA 94404

POC: Levente Egry

650-554-3447

levente.egry@lifetech.com

www.lifetechnologies.com
System Cost: N/A
Analysis Cost: N/A
Survey Source: Vendor and Internet Supplied Information
General Description
The Applied Biosystems 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. The system is intended for laboratory analysis and is versatile and adaptable for many matrices.
Technical Description
High Throughput (HT) Real-time TaqMan® and other probe/dye polymerase chain reaction amplification of genes on a Peltier-based thermocycling apparatus.
Notes:
The model 7900 is popular when high throughput is a priority. When lower numbers of samples and lower cost is desired the model 7500 is often selected.
Evaluation Criteria
THROUGHPUT:
  • Between 60 minutes and 8 hours per assay
  • Multiple samples, multiple tests/sample per run
  • Greater than 750 samples every 2 hours
  • The system or device is currently semi-automated
  • Device or system is intended for multiple detection assays
  • 3 solutions, buffer, eluents, and/or reagents
  • 2 components
  • Less than 5 minutes is required for set-up
  • 3-5 steps are required for detection
LOGISTICS:
  • An afternoon of training and some technical skills required
  • Approximately the size of a home dishwasher
  • Weighs more than 50 kg
  • Wired connections are available
  • System or device has 220V electrical requirement
  • The device is not intended for portable use
  • Is commercially available
OPERATIONS:
  • Can be used from 4°C to 37°C
  • Components must be frozen (-20°C)
  • Yes, device or system has peak performance at normal
  • relative humidity conditions
  • Between 1 to 3 years
  • 5-10 years shelf life
  • Results can be viewed in real-time
  • The system is not capable of autonomy
  • The system software is closed and not available for modification
  • The system hardware is closed and not available for modification
DETECTION:
  • Not possible for the system to achieve 510K clearance
  • Not possible for the system to achieve FDA approval
  • Less than 50 µL per analysis
  • 1-100 CFU/mL of original sample
  • 1-100 PFU/mL of original sample
  • Manual kit not integrated with the system handles spore lysis
Tier Selection
BioImageChemImageRadImage
Field Use
Mobile
Diagnostic
Analytical
Tier Key:
Tier 1 Top Tier
Tier 2 Second Tier
Tier 3 Third Tier
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Tier 5 Bottom Tier
Scoring Analysis
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FIELD USE
MOBILE USE
DIAGNOSTIC USE
ANALYTIC USE