Pacific Northwest National Laboratory Biodetection Enabling Analyte Delivery System (BEADS) | Battelle

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Battelle - Pacific Northwest National Laboratory Biodetection Enabling Analyte Delivery System (BEADS)

Contact:

Battelle - Pacific Northwest National Laboratory

P.O. Box 999, Battelle Blvd.

Mail Stop P7-50

Richland, WA 99352

Attn: Dr. Richard M. Ozanich

richard.ozanich@pnl.gov

www.pnl.gov

System Cost: $1,000-$4,000

Analysis Cost: $10-$20

Survey Source: Vendor Supplied Information

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General Description
BEADS is a universal fluidics platform that enables automated processing of surface functionalized magnetic and non-magnetic beads. BEADS enables faster more sensitive assays for a wide range of biothreat targets including DNA/RNA, bacteria, viruses, spores, oocytes, proteins, etc. BEADS enables a high degree of sample purification and concentration and is applicable for a broad range of matrices. BEADS serves as a front-end to a variety of detectors (e.g., PCR, Flow Cytometry, Electrochemical detectors, Fluorescence or Absorbance Detectors, etc.) or can be integrated with robotic systems . BEADS can serve as a sample purification and concentration front-end, or detectors can be integrated within BEADS.

Technical Description
The key enabling technology is a bead trap/flow-cell. The flow cell isolates surface functionalized magnetic or non-magnetic beads in a mini-column, which allows sample, reagents, and wash solutions to be passed over the bead column, resulting in significantly improved mass transport and faster, more sensitive assays. The microbeads can be functionalized with a wide range of target analyte capture ligands including oligonucleotides, antibodies and lectins. Semi-selective beads (e.g., silica, hydroxyapatite) can also be used to capture a wide range of pathogens or nucleic acids utilizing electrostatic capture.

Evaluation Criteria

THROUGHPUT:

Between 2 and 15 minutes for detection
1 sample, <10 tests/sample per run
Less than 32 samples every 2 hours
The system could be adapted to a fully automated system with some effort
Device or system is intended for multiple detection assays
3 solutions, buffer, eluents, and/or reagents
1 component
Less than 5 minutes is required for set-up
1-2 steps are required for detection

LOGISTICS:

Very brief (minutes-hours) training and minimal technical skills
Approximately the size of a carry-on luggage suitcase
Between 5 and 25 kg
Satellite, wireless and wired connections are available
System or device has 110V electrical requirement
4-8 hours battery life

OPERATIONS:

Can be used from 4°C to 41°C
Components must be stored at 4°C
Performance is not influenced by relative humidity
Between 6 months and 1 year shelf life
3-5 years expected life
Results can be viewed in real-time
The system or device is currently fully autonomous
The system software is open and available for modification
The system hardware is open and available for modification

DETECTION:

Possible the system could receive 510K clearance, no current efforts at this time
Possible the system could receive FDA approval, no current efforts at this time
Less than 10 µL
Excellent specificity. System has occasional false alarms under certain conditions (<2%)
1-100 colony forming units (CFU) per mL
1-100 plaque forming units (PFU) per mL
Add on capability that is full or semi-automated for spore lysis

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