7500 Fast Real-Time PCR System | Applied Biosystems

General Description
For Research Use Only. Not for use in diagnostic procedures. ABI 7500 Fast Real-Time PCR System is used for molecular biology applications via quantitative or real-time PCR, including quantitative gene expression analysis, genotyping, SNP analysis, pathogen detection, drug target validation and for measuring RNA interference. 7500 Fast can also be used to quantify messenger RNA (mRNA) and MicroRNA (miRNA) in cells or tissues. Customers may develop/design their own assays or take advantage of Applied Biosystems/Life Technologies pre-designed, fully-optimized assays.

Technical Description
ABI 7500 Fast uses detection systems that employ real-time TaqMan® polymerase chain reaction amplification of genes on a Peltier-based thermocycling apparatus, or customer-designed SYBR-based assays.

Notes:
This device is used by the DoD and CDC LRN laboratories for real-time PCR analysis.

Evaluation Criteria

THROUGHPUT:

Between 30 and 60 minutes for detection
Multiple samples, multiple tests/sample per run
95-32 samples every 2 hours
The system could be adapted to a fully automated system with some effort
Device or system is intended for multiple detection assays
0-1 solutions, buffer, eluents, and/or reagents
3 components
No set-up of the system is required
3-5 steps are required for detection

LOGISTICS:

A day of training and technical skills are required
Approximately the size of a carry-on luggage suitcase
Between 25 and 50 kg
Wireless and wired connections are available
System or device has 110V electrical requirement
The device is not intended for portable use
Is commercially available

OPERATIONS:

Can be used from 4°C to 37°C
Components must be frozen (-20°C)
Performance is not influenced by relative humidity
Between 6 months and 1 year shelf life
3-5 years expected life
Results cannot be viewed in real-time
The system is not capable of autonomy
The system software is closed and not available for modification
The system hardware is closed and not available for modification

DETECTION:

Efforts are underway to achieve 510K clearance
Efforts are underway to achieve FDA approval
Less than 50 µL
Excellent specificity. System has occasional false alarms under certain conditions (<2%)
1-100 CFU/mL of original sample
1-100 PFU/mL of original sample
Manual kit not integrated with the system handles spore lysis

Tier Selection