General Description
The Cary Eclipse Spectrophotometer uses a Xenon flash lamp for superior sensitivity, high signal-to-noise, and fast kinetics. It measures the emission of light from samples in four modes. Using Xenon lamp technology, it captures a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts. The Cary Eclipse if the only spectrophotometer with room light immunity. With an optional microplate reader, if offers full wavelength scanning of 384 samples. A BioMelt package provides the capability of thermal denaturation studies.
Technical Description
Four collection modes: fluorescence, phosphorescence, chemi/bio-luminescence, and time resolved phosphorescence. Molecular software: application focused software for novice and expert users High Sensitivity: red-sensitive photomultiplier tube detectors extends to 900 nm Small volume samples: measures low-volume picomolar fluorescein concentrations (<0.5mL) and large, odd-sized samples. Fast kinetics: scans at 24,000 nm/min without peak shifts using Xenon flash lamp technology and up to 80 points per second in steady-state fluorescence mode. Fast reactions of 1-2 seconds can be measured using the rapid mix accessory or the stopped flow accessory. Room light immunity: the instrument operates with the front and top panels removed for easy access to sample and fast installation of large accessories.
Evaluation Criteria
THROUGHPUT:
- Detection is instantaneous
- Continuous operation with no defined runs
- System is continuous and provides real time analysis with no defined tests/samples
- The system could be adapted to a fully automated system with some effort
- Device or system is intended for multiple detection assays
- 0-1 solutions, buffer, eluents, and/or reagents
- 1 component
- Less than 5 minutes is required for set-up
- 3-5 steps are required for detection
LOGISTICS:
- Very brief (minutes-hours) training and minimal technical skills
- Approximately the size of a carry-on luggage suitcase
- Between 5 and 25 kg
- Wired connections are available
- System or device has 110V electrical requirement
OPERATIONS:
- Can be used from 4°C to 41°C
- Components must be stored at room temperature (27°C)
- Device or system has peak performance at normal relative humidity conditions
- Greater than 3 years shelf life
- Greater than 10 years expected life
- Results can be viewed in real-time
- The system could be adapted to a fully autonomous system with some effort
- The system software is closed and not available for modification
- The system hardware is closed and not available for modification
DETECTION:
- Possible the system could receive 510K clearance, no current efforts at this time
- Possible the system could receive FDA approval, no current efforts at this time
- Less than 10 µL
- Superior specificity. False alarm rate approaching zero (~0%)
- > 1x10-3 mg/m3
- 1 ppm–100 ppm
- System can currently identify aerosolized chemical agent
- System can currently identify liquid chemical agent