FluorSPR | Ciencia, Inc. | WMD Detector Selector

General Description
Ciencia's FluorSPR is a dual-mode microarray instrument platform combining a surface plasmon resonance (SPR) analyzer with a surface plasmon coupled emission (SPCE) mode. In its SPR mode it provides capabilities equal to or superior to competing SPR instruments, offering highly multiplexed measurements of high molecular weight analytes in real time. The SPCE mode produces highly directional fluorescence enabling detection in the femptomolar range. The dual-mode instrument is specifically designed to chemically capture (using antibodies, aptamers) and quantify the pattern of cytokines secreted.

Technical Description
The instrument combines Grating-coupled Surface Plasmon Resonance and Surface Plasmon Coupled Emission functionality into one instrument. Detection of all analytes present in a single sample occurs simultaneously. The specificity depends on antibody or aptamer selection and binding. Detection depends on the mass of analyte captured or an enhanced fluorescence signal.

Evaluation Criteria

THROUGHPUT:

Between 30 and 60 minutes for detection
1 sample, >10 tests/sample per run
Less than 32 samples every 2 hours
The system or device is currently semi-automated
Device or system is intended for multiple detection assays
0-1 solutions, buffer, eluents, and/or reagents
1 component
Less than 5 minutes is required for set-up
Automatic detection

LOGISTICS:

Very brief (minutes-hours) training and minimal technical skills
Approximately the size of a carry-on luggage suitcase
Between 5 and 25 kg
Wired connections are available
System or device has 110V electrical requirement

OPERATIONS:

Can be used from 4°C to 41°C
Components must be stored at 4°C
Between 6 months and 1 year shelf life
5-10 years expected life
Results can be viewed in real-time
The system is not capable of autonomy
The system software is closed and not available for modification
The system hardware is closed and not available for modification

DETECTION:

Possible the system could receive 510K clearance, no current efforts at this time
Possible the system could receive FDA approval, no current efforts at this time
Less than 100 µL
Excellent specificity. System has occasional false alarms under certain conditions (<2%)
10,000-100,000 CFU per mL
10,000-100,000 PFU per mL
10-100 ng per mL
Spore lysis not necessary for detection by system
Possible system could be adapted to identify aerosolized chemical agent
System current can identify liquid chemical agent